RADIATION-INDUCED DIFFERENTIATION OF HUMAN SKIN FIBROBLASTS - RELATIONSHIP WITH CELL-SURVIVAL AND COLLAGEN PRODUCTION

Citation
Pc. Lara et al., RADIATION-INDUCED DIFFERENTIATION OF HUMAN SKIN FIBROBLASTS - RELATIONSHIP WITH CELL-SURVIVAL AND COLLAGEN PRODUCTION, International journal of radiation biology, 70(6), 1996, pp. 683-692
Citations number
25
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
ISSN journal
09553002
Volume
70
Issue
6
Year of publication
1996
Pages
683 - 692
Database
ISI
SICI code
0955-3002(1996)70:6<683:RDOHSF>2.0.ZU;2-B
Abstract
The aim of this study was to determine whether significant inter-indiv idual differences exist between skin fibroblast strains obtained from radiotherapy patients in both radiation-induced differentiation and co llagen production in vitro, for use as potential parameters for a pred ictive assay for fibrosis following radiotherapy in patients. Morpholo gical cell differentiation was determined 7 days after irradiation in seven early-passage primary human fibroblast cell strains and correlat ed with cell survival. Collagen production was measured in two cell st rains by now cytometry and incorporation of H-3-proline. There was a w ide variation in the extent of radiation-induced differentiation for t he seven cell strains, each showing a dose-related increase. The corre lation between induced differentiation and cell survival was poor (r = 0.64) but statistically significant (p < 0.01). Collagen synthesis in creased 7 days after irradiation for one cell strain (HF-48), as measu red by incorporation of H-3-proline, but not in radiation sensitive AT -1 cells. The collagen I content of the two cell strains was assessed by now cytometry but no significant differences were observed between the strains tested or with increasing dose. In conclusion, marked vari ations in radiation-induced fibroblast differentiation were observed b etween patients, this being an important criterion for a predictive as say.