Pc. Lara et al., RADIATION-INDUCED DIFFERENTIATION OF HUMAN SKIN FIBROBLASTS - RELATIONSHIP WITH CELL-SURVIVAL AND COLLAGEN PRODUCTION, International journal of radiation biology, 70(6), 1996, pp. 683-692
Citations number
25
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
The aim of this study was to determine whether significant inter-indiv
idual differences exist between skin fibroblast strains obtained from
radiotherapy patients in both radiation-induced differentiation and co
llagen production in vitro, for use as potential parameters for a pred
ictive assay for fibrosis following radiotherapy in patients. Morpholo
gical cell differentiation was determined 7 days after irradiation in
seven early-passage primary human fibroblast cell strains and correlat
ed with cell survival. Collagen production was measured in two cell st
rains by now cytometry and incorporation of H-3-proline. There was a w
ide variation in the extent of radiation-induced differentiation for t
he seven cell strains, each showing a dose-related increase. The corre
lation between induced differentiation and cell survival was poor (r =
0.64) but statistically significant (p < 0.01). Collagen synthesis in
creased 7 days after irradiation for one cell strain (HF-48), as measu
red by incorporation of H-3-proline, but not in radiation sensitive AT
-1 cells. The collagen I content of the two cell strains was assessed
by now cytometry but no significant differences were observed between
the strains tested or with increasing dose. In conclusion, marked vari
ations in radiation-induced fibroblast differentiation were observed b
etween patients, this being an important criterion for a predictive as
say.