GENETIC AND PHYLOGENETIC CLUSTERING OF ENTEROVIRUSES

Genetic and phylogenetic analysis of enteroviruses showed that in the 5'NCR enteroviruses formed three clusters: polioviruses (PVs), coxsack ievirus A type 21 (CAV21), CAV24 and enterovirus type 70 (ENV70) forme d one cluster; coxsackievirus B isolates (CBVs), CAV9, CAV16, ENV71, e chovirus type 11 (EV11), EV12 and all partially sequenced echoviruses and swine vesicular disease virus (SVDV) belonged to another cluster a nd bovine enteroviruses (BEVs) formed the third cluster, In the capsid coding region five clusters were seen: PVs, CAV21 and CAV24 formed on e cluster (PV-like); ENV70 formed a cluster of its own; all CBVs, CAV9 , EV11, EV12 and SVDV formed the third cluster (CBV-like); CAV16, CAV2 and ENV71 belonged to the fourth cluster (CAV16-like) and BEVs formed their own cluster (BEV-like), In the 3'NCR the same clusters were see n as in the coding region suggesting a close association of the 3'NCR with viral proteins while the cellular environment may be more importa nt in the evolution of the 5'NCR, Secondary structures were predicted in the 3'NCR, which showed two different patterns among the five clust ers, A potential pseudoknot region common in all five clusters was ide ntified, Although the BEV-like viruses formed a separate cluster in al l genomic regions, in the coding region they seem to be phylogenetical ly related to the CAV16-like viruses.