THE EFFECTS OF GIBBERELLIC-ACID, PHENYLETHYLAMINE, 2,4-D, AND GENOTYPE ON ANDROGENESIS IN HEXAPLOID WHEAT (TRITICUM-AESTIVUM)

The response of three hexaploid wheats to anther culture was tested on media containing gibberellic acid (GA3) or beta-phenylethylamine (PEA ) in the presence or absence of 2,4-Dichlorophenoxyacetic acid (2,4-D) . The experimental design consisted of randomized blocks with 3 replic ations per treatment. In each treatment approximately 1000 anthers wer e cultured. Genotype, BP-40 had the highest value for the number of em bryoids per 100 anthers but almost all of the regenerated plants were albino. In contrast, genotype BP-19 produced the highest number of gre en regenerants. In two genotypes out of three studied an antagonistic effect between GA3 and 2,4-D for embryo yield was observed. Replacing 2,4-D by either gibberellic acid or beta-phenylethylamine in the mediu m decreased the number of embryoids and regenerated plantlets per 100 anthers. PEA increased the number of embryoids per 100 anthers in two genotypes. Basal medium supplemented with gibberellic acid increased t he ratio of plant regeneration in comparison with the control basal me dium in one out of three genotypes tested. Haploid plantlets were prod uced at a high frequency in induction medium supplemented with GA3 (wi th or without 2,4-D).