CHARACTERIZATION OF A 23 KDA POLYPEPTIDE INDUCED BY PHOMA-LINGAM IN BRASSICA-NAPUS LEAVES

Modifications in the pattern of proteins accumulated in Brassica napus leaves after inoculation with Phoma lingam were studied. 2-D electrop horesis revealed the accumulation of a 23 kDa, pI 7 polypeptide (P23) in the inoculated plants 24 h after inoculation. Antibodies raised in rabbits against this polypeptide were used to study its systemic induc tion. In the first leaf, a significant increase of P23 was detected in the necrosis zone, but also at a lower level in the non-infected zone . Immunodetection of P23 in protein extracts and among the products of in vitro translation showed the relationship between the appearance o f this polypeptide and stress induction during the first phase of the disease.