Ls. Greek et al., RATIONAL DESIGN OF FIBEROPTIC PROBES FOR VISIBLE AND PULSED-ULTRAVIOLET RESONANCE RAMAN-SPECTROSCOPY, Applied optics, 35(21), 1996, pp. 4086-4095
The investigated the performance of fiber-optic resonance Raman probes
with a series of experiments to determine the working curves of such
probes using model analytes and to investigate the effects of absorbin
g media. A computer model designed to simulate these experiments is pr
esented, and numerical results are found to be in agreement with the e
xperimental data. Design considerations resulting from these studies a
re discussed, and novel designs for overcoming problems of coupling ef
ficiency, damage threshold, and sensitivity in absorbing samples are p
resented. These findings are applied to the design of fiber-optic prob
es for ultraviolet resonance Raman spectroscopy involving nanosecond p
ulsed-ultraviolet excitation (225 and 266 nm). These probes have been
used to collect what is, to our knowledge, the first reported fiber-op
tic-linked ultraviolet resonance Raman spectra of tryptophan and DNA.
(C) 1996 Optical Society of America