G. Hubens et al., THE INFLUENCE OF A PNEUMOPERITONEUM ON THE PERITONEAL IMPLANTATION OFFREE INTRAPERITONEAL COLON-CANCER CELLS, Surgical endoscopy, 10(8), 1996, pp. 809-812
Background: In order to test the influence of a pneumoperitoneum on th
e peritoneal implantation of free intraperitoneal colon cancer cells,
40 male syngeneic WAG rats were at random divided into four groups. Me
thods: Group 1 (n = 10) animals underwent a midline laparotomy and 10(
4) CC531 colon cancer cells were injected intraperitoneally (IF); in g
roup 2 (n = 10) 10(4) CC531 cells were injected TP without further man
ipulation; in group 3 (n = 10) a pneumoperitoneum up to 10 mmHg was cr
eated after the IP injection of the same amount of CC531 cells, The pn
eumoperitoneum was maintained for 15 min, Finally in group 4 (n = 10)
after the IP injection of 10(4) CC531 cells and after the creation of
a pneumoperitoneum, two 14-G IV catheters simulating trocars were intr
oduced in each flank. A follow-up period of 8 weeks was used, Tumor im
plantation was scored according to the peritoneal cancer index of Egge
rmont and the index of Chauffert. Results: Tumor nodules were found va
rying from 60% in groups 1-3 to 50% in group 4, There was no statistic
al difference between the implantation rate in the four groups, A port
-site recurrence was seen in group 4; all the other tumor implants wer
e located in the mesenterium, omentum, internal genitals, or parietal
peritoneum. Conclusions: The presence of a pneumoperitoneum does not e
nhance the implantation of free intraperitoneal malignant colon cancer
cells in the rat, but the presence of a ''port'' may lead to abdomina
l-wall metastases.