SIMULTANEOUS DETERMINATION OF ISOTHIOCYANATES, INDOLES, AND OXAZOLIDINETHIONES IN MYROSINASE DIGESTS OF RAPESEEDS AND RAPESEED MEAL BY HPLC

HPLC has been used for the analysis of isothiocyanates, indoles, and o xazolidinethiones in rapeseeds and rapeseed meal. The samples were tre ated with myrosinase and the released hydrolysis products extracted wi th dichloromethane. The separation was performed on an RP-18 column us ing a gradient system with acetonitrile and water. Use of a programmab le UV detector permitted the detection of the compounds at their absor ption maxima of 210 and 240 nm, respectively. Response factors of eigh t standard compounds were calculated for 240 nm. The contents of gluco sinolates calculated with the results of this method showed a signific ant linear correlation (r = 0.9995; P < 0.005) with the contents of gl ucosinolates evaluated with the results of the HPLC method of desulfog lucosinolates.