ARE CYTOSKELETAL COMPONENTS INVOLVED IN THE CONTROL OF HEPATIC CARNITINE PALMITOYLTRANSFERASE-I ACTIVITY

The present work was undertaken to test whether cytoskeletal component s are involved in the control of rat-liver carnitine palmitoyltransfer ase I (CPT-I) activity by cellular effecters. The microtubule stabiliz er taxol abolished the changes in CPT-I activity induced by the effect ers tested. Taxol also prevented OA-induced shrinkage of hepatocytes a s well as the enhanced release of lactate dehydrogenase from digitonin -permeabilized hepatocytes. On the basis of its relative sensitivity t o tautomycin and OA, the modulation of CPT-I activity seemed to involv e mostly protein phosphatase 1. These data suggest that the short-term control of hepatic CPT-I by cellular effecters may involve modulation of interactions between CPT-I and cytoskeletal components. (C) 1996 A cademic Press, Inc.