EFFECT OF ESTROGEN-TREATED PORCINE AMPULLA OVIDUCTAL EPITHELIAL-CELLSON EARLY EMBRYONIC-DEVELOPMENT IN-VITRO AND CHARACTERIZATION OF THEIRPROTEIN SYNTHETIC ACTIVITY
P. Xia et al., EFFECT OF ESTROGEN-TREATED PORCINE AMPULLA OVIDUCTAL EPITHELIAL-CELLSON EARLY EMBRYONIC-DEVELOPMENT IN-VITRO AND CHARACTERIZATION OF THEIRPROTEIN SYNTHETIC ACTIVITY, Animal reproduction science, 45(3), 1996, pp. 217-229
Recent studies by Buhi et al. have demonstrated that estrogen (E(2)) i
s responsible for the induction of de novo synthesis and secretion of
certain oviductal secretory proteins (OSP) and inhibition of other OSP
in porcine oviductal explant cultures. The present work was undertake
n to evaluate the effect of E(2)-treated oviductal epithelial cell coc
ulture on the development of early porcine embryos derived from in vit
ro matured and fertilized oocytes. In vitro synthesis of secretory pro
teins by E(2)-treated oviductal cells used for coculture was also inve
stigated by one-dimensional (1D) and two-dimensional (2D) sodium dodec
yl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE). The result
s showed that the cleavage rate was significantly enhanced by cocultur
ing fertilized eggs with E(2)-treated oviductal epithelial cells. The
in vitro protein synthetic pattern of oviductal secretory proteins was
influenced by E(2) treatment. These variations included the disappear
ance of one protein (82 000 M(r)) and the appearance of another (33 00
0 M(r)) in the E(2)-treated group as assessed by 1D-SDS-PAGE. Addition
al proteins of M(r) 97 000 and an M(r) 36 000-45 000 complex were incr
eased in abundance by the E(2) treatment. Analyses by 2D-SDS-PAGE reve
aled three major E(2)-dependent proteins, of M(r) 45 000 (pI 5.5), 43
000 (pI 5.5) and a 36 000-45 000 M(r) (pI 4.8) protein complex, wherea
s polypeptides of M(r) 97 000 (pI 5.1), 36 000 (pI 8.0) and 25 000 (pI
6.8) were inhibited by E(2) treatment. The results demonstrated that
porcine epithelial cell protein synthetic patterns are influenced by E
(2) treatment and that estradiol treatment of oviductal cells may incr
ease the rate of zygote cleavage during early development in vitro in
pigs.