Hyperpolarized Xe-129 has been used to obtain gas phase images of mous
e lung in vivo, showing distinct ventilation variation as a function o
f the breathing cycle. Spectra of Xe-129 in the thorax show complex st
ructure in both the gas phase (-4 to 3 ppm) and tissue-dissolved (190-
205 ppm) regions. The alveolar gas peak shows correlated intensity and
frequency oscillations, both attributable to changes in lung volume d
uring breathing. The two major dissolved peaks near 195-200 ppm are at
tributed to lung parenchyma and to blood; they reach maximum intensity
in 5-10 s and decay with an apparent T-1 of 30 s. Another peak at 190
ppm takes 20-30 s to reach maximum; this must represent other well-va
scularized tissue (e.g., heart and other muscles) in the thorax. The m
aximum integrated area of the tissue components reaches 30-80% of the
maximum alveolar gas area, indicating that imaging at tissue frequenci
es can be achieved.