THE IGF-I RECEPTOR GENE PROMOTER IS A MOLECULAR TARGET FOR THE EWINGS-SARCOMA WILMS-TUMOR-1 FUSION PROTEIN

Desmoplastic small round cell tumor (DSRCT) is an abdominal malignancy in children which is characterized by a recurrent chromosomal translo cation, t(11; 22)(p13;q12). This rearrangement results in the fusion o f the ubiquitously expressed EWS1 gene to the Wilms' tumor suppressor (WT1) gene. The chimeric protein contains the N-terminal domain of EWS 1 fused to the DNA-binding domain of WT1, including zinc fingers 2-4, Because WT1 has been shown previously to bind and repress the insulin- like growth factor I (IGF-I-R) promoter, we investigated whether this promoter is, in addition, a target for the aberrant EWS/WT1 transcript ion factor. EWS/WT1 activated the ICF-I-R. promoter similar to 340%, w hereas a fusion protein containing a three-amino acid insert (KTS) bet ween zinc fingers 3 and 4 had no effect. On the other hand, expression vectors encoding either WT1 or EWS1 reduced the activity of the promo ter to 46 and 58% of control values, respectively. Results of gel shif t assays indicate that the binding affinity of EWS/WT1 to a fragment o f the 5'-flanking region of the receptor promoter was higher than the affinity of WT1 itself. Consistent with the results of functional assa ys, the binding of EWS/WT1(+KTS) was significantly reduced. Due to the central role of the IGF-I-R in tumorigenesis, activation of the recep tor promoter by EWS/WT1 may constitute a potential mechanism for the e tiology and/or progression of DSRCT.