Starch gel electrophoresis revealed that the alcohol dehydrogenase (AD
H-2) locus was polymorphic in two populations (from Agua Caliente, Cal
ifornia and the Grand Canyon, Arizona) of cactophilic Drosophila mojav
ensis that utilize barrel cactus (Ferocactus acanthodes) as a host pla
nt. Electromorphs representing products of a slow (S) and a fast (F) a
llele were found in adult flies. The frequency of the slow allele was
0.448 in flies from Agua Caliente and 0.659 in flies from the Grand Ca
nyon. These frequencies were intermediate to those of the low (Baja Ca
lifornia peninsula, Mexico) and high (Sonora, Mexico and southern Ariz
ona) frequency Adh-2(s) populations of D. mojavensis that utilize diff
erent species of host cacti.