Lz. Jin et al., EFFECT OF ADHERENT LACTOBACILLUS SPP ON IN-VITRO ADHERENCE OF SALMONELLAE TO THE INTESTINAL EPITHELIAL-CELLS OF CHICKEN, Journal of Applied Bacteriology, 81(2), 1996, pp. 201-206
Single strains of Lactobacillus acidophilus and Lact. fermentum, isola
ted from chicken intestine, were used to study in vitro interactions w
ith Salmonella enteritidis, Salm. pullorum or Salm. typhimurium in an
ileal epithelial cell (IEC) radioactive assay. Exclusion, competition
and displacement phenomena were investigated by respectively incubatin
g (a) lactobacilli and IEC together, prior to addition of salmonellae,
(b) lactobacilli, IEC and salmonellae together, and (c) salmonellae a
nd IEC, followed by the lactobacilli. Lactobacilli were selected for s
tudy because of their strong ability to adhere to IEC and poor aggrega
tion with salmonellae. The results demonstrated that Lact. acidophilus
significantly reduced (P < 0.05) the attachment of Salm. pullorum to
IEC in the tests for exclusion and competition, but not in the displac
ement tests. Lactobacillus fermentum was found to have some ability to
reduce the attachment of Salm. typhimurium to IEC under the condition
s of exclusion (P < 0.08), competition (P < 0.09), but not displacemen
t. However, both Lact. acidophilus and Lact. fermentum were unable to
reduce the adherence of Salm. enteritidis to IEC under any of the cond
itions.