THE EFFECT OF EARLY EXPOSURE TO VIBRIO-PELAGIUS ON THE AEROBIC BACTERIAL-FLORA OF TURBOT, SCOPHTHALMUS-MAXIMUS (L) LARVAE

Vibrio pelagius was added to filtered sea water in experimental tanks containing newly-hatched larvae of Scophthalmus maximus. The bacterial load of larvae increased from day 1 post-hatch and by day 14 had reac hed 5 x 10(4) bacteria per larva. Vibrio pelagius dominated the aerobi c bacterial flora of larvae exposed to this bacterial species but was not detected in larvae not exposed to exogenous bacteria. An enzyme-li nked immunosorbent assay using rabbit antiserum against V. pelagius al lowed a specific quantitative assay for homologous bacterial antigens in individual larvae, with very little cross-reaction against heterolo gous bacteria. The results of enzyme-linked immunosorbent assays of V. pelagius antigens in larvae from tank water inoculated with this bact erial species correlated with the bacterial levels found in the larvae by culture on agar plates.