SIMULTANEOUS ANALYSIS OF ENANTIOMERIC AMINO-ACIDS BASED ON DERIVATIZATION WITH (-2-T-BUTYL-2-METHYL-1,3-BENZODIOXOLE-4-CARBOXYLIC ACID AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY())

The fluorescent chiral derivatizing agent in the title, (S)-TBMB carbo xylic acid, was applied for highly sensitive HPLC analysis of enantiom eric amino acids. Enantiomeric amino acids derivatized with the agent could be separated as the diastereomers on ODS (C-18) column and deter mined in less than 0.05 picomole amounts owing to the strong fluoresce nt chromophore of the reagent (Ex=310 nm, Em=380 nm). Three types of H PLC conditions with different pH values were proposed for the group an alysis of amino acids: pH=5.7 for the simultaneous analysis of all ami no acids, pH=4.6 for the selective analysis of polar amino acid deriva tives and pH=6.0 for that of less polar ones.