EXPERIMENTAL AND THEORETICAL ANALYSES ON FACTORS AFFECTING THE PERFORMANCE OF POLYPYRROLE GLUCOSE OXIDASE BIOSENSOR - PERMEABILITY ASPECT OF POLYPYRROLE FILM/

The effects of film thickness and enzyme concentration in the electrod eposition solution on the analytical performance of polypyrrole/glucos e oxidase (PPy/GOD) biosensor were investigated. The optimum film thic kness which maximizes the catalytic current of the electrode was obser ved at a given enzyme concentration, and this optimum thickness moved down to a thinner region with increasing concentration of enzyme. On t he basis of the above results, the conditions for electrochemical depo sition of the polypyrrole films were optimized. Theoretical analyses w ere conducted to elucidate the experimental results. The effect of ele ctrodeposition conditions on the analytical performance of the PPy/GOD biosensor was evaluated in terms of response characteristics, sensiti vity, and operational stability.