Four new botcinolide derivatives 4-O-methylbotcinolide, 3-O-acetyl-5-O
-methylbotcinolide, 3-O-acetyl-2-epibotcinolide and 2-epibotcinolide h
ave been isolated from the plant pathogen, Botrytis cinerea. Their str
uctures have been established by extensive spectroscopic methods.