VARIATION IN SALMONELLA CORE LIPOPOLYSACCHARIDE AS DETECTED BY THE MONOCLONAL-ANTIBODY M105

The lipopolysaccharide antigenicity of 22 Salmonella strains (represen ting nine serogroups) and four nonsalmonellae Enterobacteriaceae to th e Salmonella genus specific monoclonal antibody M105 was analysed. The monoclonal antibody M105 reacted with all 22 Salmonella strains. Prob ing SDS-PAGE separated LPS molecules with MAb M105 revealed that the a ntibody reacted with the core region of all Salmonella serovars, Howev er, no reaction ts as obtained to the long-chain LPS of serovars O (Sa lm. adelaide and Salm. ealing), C-1 (Salm. infantis, Salm. livingstone and Salm. virchow) or Salm. arizonae, It is plausible that the presen ce of a second core antigenic type results in the lack of reaction bet ween long-chain LPS and the Salmonella genus specific monoclonal antib ody M105.