LEUCONOSTOC-MESENTEROIDES SUBSP MESENTEROIDES FR52 SYNTHESIZES 2 DISTINCT BACTERIOCINS

Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides s ubsp. mesenteroides FR52, was purified from producing cells by the ads orption-desorption method, combined with reverse-phase high-performanc e liquid chromatography, The elution profile revealed the presence of two inhibitory peaks of activity, each displaying different inhibitory spectra. Mesenterocin 52A possessed a broad inhibitory spectrum, incl uding anti-listeria activity, while Mesenterocin 52B was only active a gainst. Leuconostoc spp. The amino acid sequence and M(r) of Mesentero cin 52A appeared identical to the previously described Mesentericin Y1 05, In contrast, Mesenterocin 52B possessed a M(r) of 3446 Da, corresp onding to 32 amino acids and a sequence that shared no homology with k nown bacteriocins: NH2-KGVLGWLSMASSALTGPQQPNSPWLAKIKNHK.