THE CAPACITY OF MURINE EPITHELIAL-CELL LINES WITH DIFFERENT DEGREES OF MALIGNANT TRANSFORMATION TO METABOLIZE BENZO[A]PYRENE

Cultures of three murine cell lines derived from fetal mouse liver (FL , FLT3 and FLT5) were treated with benzo[a]pyrene to evaluate their ab ility to activate promutagens. DNA adducts were detected in all three cell lines when they were treated with the major active metabolite of benzo[a]pyrene, benzo[a]pyrene diolepoxide. DNA adducts were also prod uced in FL and FLT3 cells when they were treated with the promutagen, benzo[a]pyrene, while treatment of FLT5 cells resulted in very few DNA adducts. In treated FL and FLT3 cells, the most evident adduct spot d etected showed a chromatographic position similar to that of the main adduct formed in cells treated with (+/-)-r-7,t-8-dihydroxy-t-9,10-oxy -7,8,9, 10-tetrahydrobenzo[a]pyrene.