SEQUENCE-ANALYSIS OF A PARVOVIRUS B19 ISOLATE AND BACULOVIRUS EXPRESSION OF THE NONSTRUCTURAL PROTEIN

Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood dona tions. We have determined the sequence of the genome of one such isola te (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, where it is more similar to the ter minus of another isolate (Ala/Alb). Recombinant nonstructural protein, NS, was constructed. Antibodies to NS, as well as to the capsid prote ins, VP1/2, were detected in patients with B19 infection.