NOVEL MEASUREMENT OF HYPOXANTHINE IN FISH USING DIRECT MEASUREMENT PROBE AND CHEMILUMINESCENCE FLOW-INJECTION ANALYSIS

Direct measurements of hypoxanthine (Hx) in fish flesh were carried ou t using a sardine and a grunt twice with the same fish, and the averag e of 10 successive measurements (from 120 sec to 480 sec after pressin g the probe against fish tissue) was taken as the Hx concentration by direct measurement. Results with two nearby tissues of one sample were 42.9 +/- 4.9 mu M and 42.6 +/- 2.6 mu M for the sardine, and 639 +/- 33 mu M and 598 +/- 29 mu M for the grunt. For sardine, Hx results fro m this direct method did not agree well with perchloric acid extractio n measurements, but they were much better for grunt.