DETERMINATION OF PEPTIDOGLYCAN-ASSOCIATED PROTEIN IN ESCHERICHIA-COLINCIB-8545 BY CAPILLARY ZONE ELECTROPHORESIS

An efficient reliable and sensitive capillary zone electrophoresis ass ay for the six major bacterial peptidoglycan-associated proteins of Es cherichia coli NCIB 8545 is described. The method provides the facilit y to determine quantitatively the effect of antibacterials on bacteria l peptidoglycan-associated protein synthesis and thus to further eluci date the mechanism of antibacterial action of such drugs as the antifo lates which recently have been shown to adversely affect peptidoglycan synthesis.