DIFFERENTIAL EXPRESSION OF LAMININS AND THEIR INTEGRIN RECEPTORS IN DEVELOPING AND ADULT HUMAN LUNG

Laminins (Ln) appear to play an important role in the morphogenesis of airways. We studied the expression of different laminin chains and th eir integrin receptors in fetal and adult lung by immunohistochemistry . Special attention was focused on the changes in the expression of th ese proteins during the development from the pseudoglandular (PG) and canalicular stages to adult lung, and on the possible implications of the changes for the normal lung development. The most significant chan ges in the expression pattern were found during the development from t he PG stage to the canalicular stage. Basement membranes (BM) of both the epithelial buds and the becoming bronchi showed reactivity for Ln- alpha 1, -alpha 3, and -beta 3 chains at all stages. The alpha 2 chain was expressed only in the epithelial buds at the PG stage, and could not be found in any epithelial structures at the canalicular stage. Si milarly, at the PG stage the Ln-beta 2 chain was expressed in BMs of b oth epithelial buds and bronchi but disappeared from the bronchial BM before the canalicular stage. Ln-beta 1 chain appeared in the bronchia l BM first in the mature lung, which suggests the presence of uncharac terized Ln-beta chains earlier in development. There were considerable changes in the expression of integrins (Int) comcomitantly with alter ations in the composition of the BMs. At the PG stage the epithelial b uds showed reactivity for Int-alpha(2), -alpha(3), and -alpha(6) subun its, but at the canalicular stage the Int-alpha(2) and -alpha(6) subun its disappeared, and only Int-alpha(3) integrin subunit was found in e volving alveolar walls; Int-alpha(6) was found in capillaries. A simil ar distribution of Int subunits was also found in adult alveoli. The b ronchi expressed Int-alpha(2) -alpha(3) and -alpha(6) subunits at all developmental stages, but the Int-beta(4) subunit emerged first at the canalicular stage. Our results suggest that there are major changes i n the expression of Ln and their Int receptors during morphogenesis of the lung, which may be important for normal development.