CELL-SEPARATION FROM HIGH CELL-DENSITY BROTHS OF ALCALIGENES-EUTROPHUS BY USING A COAGULANT

Authors
RYU HW CHO KS CHANG YK CHANG HN
Citation
Hw. Ryu et al., CELL-SEPARATION FROM HIGH CELL-DENSITY BROTHS OF ALCALIGENES-EUTROPHUS BY USING A COAGULANT, Biotechnology techniques, 10(12), 1996, pp. 899-904
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
12
Year of publication
1996
Pages
899 - 904
Database
ISI
SICI code
0951-208X(1996)10:12<899:CFHCBO>2.0.ZU;2-D
Abstract
Alcaligenes eutrophus was successfully recovered from high cell densit y broths by pre-treatment with polyaluminium hydroxide chloride silica te as a coagulant at 36 - 90 mg Al/l. The optimum pH range for cell co agulation was 10-12. Subsequent centrifugation (45 x g) and filtration (pore size 0.5 mm) gave a cell recovery of higher than 90%. The energ y demand for cell recovery with the coagulant was only 3-11% of that w ithout it.