CHEMICAL STABILIZATION OF RECOMBINANT HORSERADISH-PEROXIDASE

Authors
OBRIEN AM FAGAIN CO
Citation
Am. Obrien et Co. Fagain, CHEMICAL STABILIZATION OF RECOMBINANT HORSERADISH-PEROXIDASE, Biotechnology techniques, 10(12), 1996, pp. 905-910
Citations number
19
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
12
Year of publication
1996
Pages
905 - 910
Database
ISI
SICI code
0951-208X(1996)10:12<905:CSORH>2.0.ZU;2-C
Abstract
Unglycosylated recombinant horseradish peroxidase (HRP C) had a half life of 21 minutes at 65 degrees C compared with only 5 minutes for th e plant enzyme (HRP). The half life of HRP C at 65 degrees C increase d by 5-fold following modification with ethylene glycol bis(succinic a cid N-hydroxysuccinimide ester). Tolerance to 60% 1,4-dioxan also incr eased whilst tolerance to 30% dimethylformamide was unchanged.