TRANSFORMATION OF PHAFFIA-RHODOZYMA BY ELECTROPORATION

Authors
RUBINSTEIN L ALTAMIRANO A SANTOPIETRO LD BAIGORI M DEFIGUEROA LC
Citation
L. Rubinstein et al., TRANSFORMATION OF PHAFFIA-RHODOZYMA BY ELECTROPORATION, Biotechnology techniques, 10(12), 1996, pp. 929-932
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
12
Year of publication
1996
Pages
929 - 932
Database
ISI
SICI code
0951-208X(1996)10:12<929:TOPBE>2.0.ZU;2-A
Abstract
The transformation of Phaffia rhodozyma by electroporation was better than the one by the lithium chloride (LiCl) or spheroplast transformat ion methods. The influence of several parameters on transformation eff iciency was studied. Electroporation conditions of 1200 Volts, 50 mu F arads and 2000 Ohms were proved successful. A maximum of 1.5 x 10(3) t ransformants per 100 ng of DNA was reached.