GROWTH-STIMULATION WITH HONEY ROYAL JELLY DIII PROTEIN OF HUMAN LYMPHOCYTIC CELL-LINES IN A SERUM-FREE MEDIUM

Authors
WATANABE K SHINMOTO H KOBORI M TSUSHIDA T SHINOHARA K KANAEDA J YONEKURA M
Citation
K. Watanabe et al., GROWTH-STIMULATION WITH HONEY ROYAL JELLY DIII PROTEIN OF HUMAN LYMPHOCYTIC CELL-LINES IN A SERUM-FREE MEDIUM, Biotechnology techniques, 10(12), 1996, pp. 959-962
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
12
Year of publication
1996
Pages
959 - 962
Database
ISI
SICI code
0951-208X(1996)10:12<959:GWHRJD>2.0.ZU;2-N
Abstract
The growth stimulating effects of a royal jelly protein (DIII protein) were studied. The DIII protein stimulated the growth of five human ly mphocytic cell lines in serum-free conditions, Cell cycle analysis sho wed that U-937 cells cultured with the DIII protein did not arrest to the Ct phase, Furthermore, a binding assay using europium-labeled DIII protein showed U-937 cells had a large number of low affinity recepto rs on the cell surface.