T. Mewes et al., INCREASED IN-VITRO CYSTEINYL LEUKOTRIENE RELEASE FROM BLOOD LEUKOCYTES IN PATIENTS WITH ASTHMA, NASAL POLYPS, AND ASPIRIN INTOLERANCE, Allergy, 51(7), 1996, pp. 506-510
In vitro cysteinyl leukotriene (cLT) release from blood leukocytes was
measured in eight normal individuals (NI), nine patients with nasal p
olyps CNP) without aspirin intolerance, and eight patients with NP. as
thma, and aspirin intolerance CAI), Blood leukocytes were prestimulate
d with interleukin-3 (IL-3) and incubated with acetylsalicylic acid (A
SA) (10 and 100 mu g/ml) together with C5a (10(-8) mol/l) for 18 h, cL
T release (LTC(4), LTD(4) and LTE(4)) from blood leukocytes was measur
ed with a competitive enzyme-linked immunoassay. Background cLT releas
e was 259+/-66 pg/ml (mean+/-SEM) in the NI group, 185+/-33 pg/ml in t
he NP group, and 578+/-136 pg/ml in the AI group (P=0.1). After incuba
tion with 10 mu g/ml ASA, cLT concentration was lower in normal subjec
ts (346+/-72 pg/ml) and in patients with NP (209+/-53) than in patient
s with AI (686+/-75 pg/ml, P=0.002). After incubation with 100 mu g/ml
ASA, cLT concentrations were 285+/-72 pg/ml in the NI group, 313+/-77
pg/ml in the NP group, and 654+/-121 pg/ml in the AI group (P=0.04),
respectively., Simultaneous incubation with ASA 10 mu g/ml and C5a (10
(-8)mol/l resulted in a cLT concentration of 751+/-171 pg/ml in the NI
group, 343+/-102 pg/ml in the NP group, and 2196+/-480 pg/ml in patie
nts with AI (P=0.0006), whereas simultaneous incubation with ASA 100 g
/ml and C5a (10(-8)mol/l) resulted in 268+/-51 pg/ml in the NI group,
412+/-97 patients with AI, cLT release from blood leukocytes is altere
d when compared with normals and patients with NP. The presented cLT-r
elease assay could be of potential use in the in vitro diagnosis of AT
.