The hydrolysis of gelatinized commercial barley starch by Bacillus lic
heniformis alpha-amylase began by rapid depolymerization of amylopecti
n in two stages. The formation of low molecular weight hydrolysis prod
ucts, as indicated by an increase in reducing power, was only observed
when all the original high molecular weight amylopectin had degraded
further. The hydrolysis of waxy barley starch followed a similar patte
rn. Hydrolysis had a big influence on the microstructure of the starch
dispersion: first the degradation of the amylopectin rich granule res
idues ('ghosts') was observed, and later amylose aggregates were forme
d.