CLEAVAGE OF OLIGODEOXYRIBONUCLEOTIDES FROM CONTROLLED-PORE GLASS SUPPORTS AND THEIR RAPID DEPROTECTION BY GASEOUS AMINES

A novel method for the deprotection of oligodeoxyribonucleotides has b een developed. Gaseous amines such as ammonia or methylamine were empl oyed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tent-butyl)phenoxyac etyl group for the protection of the exocyclic amino function of cytos ine, adenine and guanine were released from controlled-pore glass supp orts and fully deprotected by ammonia or methylamine under gas phase c onditions, at room temperature, within 35 or 2 min respectively.