SYNTHESIS, CLONING AND EXPRESSION OF GENES FOR ANTIBACTERIAL PEPTIDES- CECROPIN, MAGAININ AND BOMBININ

Authors
MARTEMYANOV KA SPIRIN AS GUDKOV AT
Citation
Ka. Martemyanov et al., SYNTHESIS, CLONING AND EXPRESSION OF GENES FOR ANTIBACTERIAL PEPTIDES- CECROPIN, MAGAININ AND BOMBININ, Biotechnology letters, 18(12), 1996, pp. 1357-1362
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
12
Year of publication
1996
Pages
1357 - 1362
Database
ISI
SICI code
0141-5492(1996)18:12<1357:SCAEOG>2.0.ZU;2-C
Abstract
Three DNA sequences encoding the antimicrobial peptides bombinin, cecr opin and magainin were synthesised. DNA fragments were cloned into pET -21d plasmid under T7 promoter for expression in vivo and in vitro and into pRIT-2T plasmid for expression as a fusion product with protein A. The polypeptides synthesised in both systems possess antibacterial activity.