IMMUNOHISTOCHEMICAL LOCALIZATION OF THE SEROTONIN 5-HT2B RECEPTOR IN MOUSE GUT, CARDIOVASCULAR-SYSTEM, AND BRAIN

We recently reported the cloning of a new member of the serotonin 5-HT 2 family, the 5-HT2B receptor. We now report the production and charac terisation of a specific antiserum directed against the C-terminal por tion of the mouse 5-HT2B receptor. After affinity purification, this p olyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intesti ne and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.