A MODEL PHAGE DISPLAY SUBTRACTION METHOD WITH POTENTIAL FOR ANALYSIS OF DIFFERENTIAL GENE-EXPRESSION

In order to establish a subtractive procedure that makes it possible t o enrich selectively phage displayed antibodies directed against prote ins constituting a difference between two populations of cells, a comp etitive selection strategy utilising two solid phases was developed an d tested, Antibodies recognising a defined difference between two othe rwise identical protein mixtures were isolated and their specificity c onfirmed, To test further the efficacy of selection inhibition during the competitive selections, selections towards a total cell extract we re performed with and without competition from the same extract. An an alysis of the resulting phage antibodies confirmed the subtractive nat ure of the system described.