Motion and deformation of an object may be quantified by following att
ached markers in video or cine frame sequences. When recording cardiac
motion by video (256 x 256 pixels, 50 Hz), generally no more than app
roximately 20 markers can be followed due to difficulties in proper id
entification of marker images. In the present study we developed the l
ower rank (LR) tracking method which can automatically follow consider
ably more than 20 markers. The performance of the method was evaluated
in computer simulations of naturally moving myocardial markers observ
ed in a sequence of 60 video frames. White noise was added to the mark
er coordinates. Realistic loss of data due to detection failure was si
mulated by deleting a generated marker image when the distance to anot
her marker image was below a given minimum value. In a test, realistic
values were substituted for the noise level sigma (0.5 pixel) and the
minimum marker distance d(m) (4 pixels). For numbers of markers rangi
ng from 50 to 100, 95-90% of the detected marker images was correctly
tracked. Less than 0.7% was part of a false track, i.e. a track contai
ning images of different markers. Under less favourable conditions (si
gma = 1 pixel; d(m) = 8 pixels) the method was robust: for 75 markers
with 40% of the marker images missing, still 70% of the detected image
s was correctly tracked, while the fraction in false tracks did not in
crease. The LR tracking method appears reliable for automatic tracking
of large amounts of moving markers in a sequence of video or cine fra
mes. Copyright (C) 1996 Elsevier Science Ltd.