It has been recommended that samples submitted for microbiological exa
mination should be retained far 48 hours after issue of the final repo
rt. In order to ascertain whether reproducible results could be achiev
ed following storage of sputum specimens, two laboratories each re-cul
tured 100 samples 48 hours after their report had been issued and a fu
rther laboratory re-cultured 100 samples 48 hours after receipt, Disco
rdant results were obtained in only 5-25% of specimens, indicating tha
t potential respiratory pathogens could survive storage.