DIRECT CDNA SELECTION WITH DNA MICRODISSECTED FROM MOUSE CHROMOSOME-16 - ISOLATION OF NOVEL CLONES AND CONSTRUCTION OF A PARTIAL TRANSCRIPTION MAP OF THE C3-C4 REGION

A group of cDNA segments was selected by direct hybridization of mouse cerebellar cDNAs against genomic DNA pools generated by microdissecti on of the mouse chromosome 16 (MMU16) C3-C4 region. After elimination of repetitive sequences and adjustment for redundancy among clones, 34 novel cDNA fragments were isolated. The MMU16 origin of clones was co nfirmed by genetic linkage mapping, Reverse transcription PCR indicate d that similar to 68% of the cDNAs represent transcripts that are expr essed in adult mouse cerebellum. Northern blotting showed that some of these are predominantly or solely expressed in brain. This work demon strates that DNA microdissected from banded MMU16 can be used for dire ct cDNA selection, thus enabling construction of a new, region-specifi c partial transcription map. This selected cDNA library should be a us eful reagent for further molecular neurobiological studies.