GLUCOAMYLASE ABSORPTION AND DESORPTION PROCESS

This paper reports the study of glucoamylase absorption and desorption processes on spherical particles constituting acrylic supports. The k inetic (reaction order, half-life of the reaction, reaction rate const ant), and thermodynamic parameters (activation energy, pre-exponential factor) of the glucoamylase immobilization reaction dynamics inside t he acrylic supports have been studied. Hindrance of the penetrants (pr otein molecules), which defeats the polymer's cohesive forces and pass es through the diffusional barriers towards the sites available to rec eive it, denotes the influence of the diffusion phenomena, and might b e expressed by the values of the effective diffusion coefficient and o f the energetic parameters, as well. The desorption dynamics of glucoa mylase in specific buffer concentration gradients and temperature are dependent on the eluent volume and on the protein eluate.