STRUCTURE AND CHROMOSOMAL ASSIGNMENT OF THE HUMAN S1-5 GENE (FBNL) THAT IS HIGHLY HOMOLOGOUS TO FIBRILLIN

The human S1-5 gene (fibrillin-like; FBNL) was originally isolated fro m a subtractively enriched cDNA library established from a subject wit h Werner syndrome (WS). We isolated genomic clones containing the enti re S1-5 gene and determined its genomic structure including the exon-i ntron organization. The gene spanned approximately 18 kb of genomic DN A and consisted of 12 exons. Its expression was abundant in all tissue s examined except brain and peripheral leukocytes, where it was undete ctable. In addition, we have mapped S1-5 by fluorescence in situ hybri dization to chromosome 2p16, a position that excludes it as a candidat e for WS. Our data should facilitate an understanding of the function and regulation of S1-5 in human tissues. (C) 1996 Academic Press, Inc.