EVIDENCE OF A ROLE FOR NAD(-GLYCOHYDROLASE AND ADP-RIBOSYLTRANSFERASEIN GROWTH AND DIFFERENTIATION OF STREPTOMYCES-GRISEUS NRRL B-2682 - INHIBITION BY M-AMINOPHENYLBORONIC ACID())
A. Penyige et al., EVIDENCE OF A ROLE FOR NAD(-GLYCOHYDROLASE AND ADP-RIBOSYLTRANSFERASEIN GROWTH AND DIFFERENTIATION OF STREPTOMYCES-GRISEUS NRRL B-2682 - INHIBITION BY M-AMINOPHENYLBORONIC ACID()), Microbiology, 142, 1996, pp. 1937-1944
m-Aminophenylboronic acid (APBA) inhibited the germination, growth and
sporulation of Streptomyces griseus NRRL B-2682 in an age- and concen
tration-dependent manner in submerged and solid cultures. When added t
o cells or cell extracts it irreversibly inhibited NAD(+)-glycohydrola
se and ADP-ribosyltransferase activity. ADP-ribosyltransferase was mor
e sensitive, but inhibition was not complete, even in the presence of
10 mM APBA. The in vivo effects of the inhibitor correlated with its i
n vitro effect on ADP-ribosylation and on the profile of ADP-ribosylat
ed endogenous proteins. The physiological importance of ADP-ribosyltra
nsferase was supported by the observation that APBA strongly inhibited
the growth of a non-sporulating and NAD(+)-glycohydrolase-negative mu
tant of the parental strain. The resistance of S. griseus NRRL B-2682
strains able to grow in the presence of APBA was due to permeability f
actors. A comparison of the ADP-ribosylated protein profiles of S. gri
seus NRRL B-2682 grown under various conditions showed similarities, b
ut also specific differences. The results suggest that the ADP-ribosyl
transferase of S. griseus NRRL B-2682 is an indispensable enzyme for g
rowth and differentiation of the strain. It may regulate the activity
of key enzymes or developmental proteins by responding to intra- and e
xtracellular conditions.