Ten yeast isolates were studied as potential agents for biocontrol of
yam leaf spot, caused by Curvularia eragrostidis. Under greenhouse con
ditions most of the isolates showed good disease control. Application
of the antagonists simultaneously as C. eragrostidis inoculation gave
the best results, when the LD-18 isolate (Rhodotorula glutinis) reduci
ng disease severity reduction better than mancozeb. The efficiency of
the LD-18 was reduced by adding peptone or dextrose to eel suspensions
. Ultrasctrutural analysis of the interactions between the C. eragrost
idis and LD-18 on yam leaf surface showed conidial germination 6 hours
after inoculation, and adhesion of yeast cells to the germination tub
es. In laboratory tests, the production of non-volatile extracellular
metabolites by LD-19 could not be detected.