APPLICABILITY OF CAPILLARY ELECTROPHORESIS TO THE STUDY OF BOVINE BETA-LACTOGLOBULIN POLYMORPHISM

Capillary electrophoresis performed on uncoated capillaries is used to quantitate major whey proteins in samples of raw bovine milk from the Fleckvieh, Holstein and Menorquina breeds. The influence of capillary conditioning between runs and of sample preparation steps on reproduc ibility and resolution are discussed. No interferences from immunoglob ulin G and lactoferrin in the determination of beta-lactoglobulin and alpha-lactalbumin are demonstrated. Study of the dependence of beta-la ctoglobulin and alpha-lactalbumin concentrations on beta-lactoglobulin genotype shows no direct influence of this genotype on alpha-lactalbu min content, while the highest rate of biosynthesis of beta-lactoglobu lin is associated with allele A of this protein.