A. Vianello et al., ROLE OF PPI AND H-PPIASE IN MAINTAINING VACUOLAR PROTON GRADIENT IN METABOLIC INHIBITOR-TREATED CELLS(), Acta phytopathologica et entomologica Hungarica, 30(1-2), 1995, pp. 15-19
In Acer pseudoplatanus cells, the role of ATP and PPi in keeping the v
acuolar Delta pH was investigated after treatments with 2-deoxy-D-gluc
ose, inhibitor of glycolysis, and KCN, inhibitor of respiration, in or
der to simulate a condition of glucose startvation and anoxia. It was
concluded that the tonoplast H+-PPiase is responsible for the maintena
nce of vacuolar Delta pH and that this enyme is the major consumer of
PPi in metabolic inhibitor-treated cells.