PREPARATION OF LIPASE-SURFACTANT COMPLEX FOR THE CATALYSIS OF TRIGLYCERIDE HYDROLYSIS IN HETEROGENEOUS REACTION SYSTEMS

To facilitate lipase (E.C.3.1.1.3) catalyzed hydrolysis reaction of tr iglycerides in heterogeneous reaction systems, lipase-surfactant compl ex (LSC) was prepared by mixing an aqueous solution of lipase and surf actant in ethanol. The LSC prepared from lipase MF-30 derived from Pse udomonas sp. and Nonion SP-60R (sorbitan mono-stearate) showed a high LSC yield and activity recovery. About 30% of the protein in the crude lipase was recovered in the LSC precipitate, and further addition of surfactant to the supernatant was ineffective. Our preparation method for the selective separation of the catalytic active protein from a cr ude enzyme was suggested.