ENHANCING THE EFFECT OF ANTICANCER DRUGS AGAINST THE COLORECTAL-CANCER CELL-LINE WITH ELECTROPORATION

Electroporation was applied in vitro and in vivo in the treatment of h uman colorectal cancer cell lines to study whether it can enhance the effect of bleomycin (BLM), 5-fluorouracil (5-FU) and cia-platinum (CDD P). We used LS174T and Colo320 cells derived from human colon cancer a s target cells in this study. When the LS174T cells were used as targe t cells, the IC50 of BLM decreased to 10(-3) times, while that of 5-FU decreased to only about one fifth with the application of electric cu rrent. In the case of the Colo320 cells, the IC,, of BLM and 5-FU were about one hundredth and a half, respectively. The effect of CDDP was not enhanced with electric current. In vivo experiments were also perf ormed using LS174T cells transplanted subcutaneously (s.c.) into nude mice. By treatment with intravenously (i.v.) administrated BLM and sim ultaneous application of the electric current, tumors were markedly de creased in size after three weeks.