ISOLATION AND MOLECULAR ANALYSIS OF THE GENE FOR CYTOCHROME C(1), FROM KLUYVEROMYCES-LACTIS

Authors
GBELSKA Y HORVATHOVA K VANDERAART QJM ZONNEVELD BJM DESTEENSMA QJM SUBIK J
Citation
Y. Gbelska et al., ISOLATION AND MOLECULAR ANALYSIS OF THE GENE FOR CYTOCHROME C(1), FROM KLUYVEROMYCES-LACTIS, Current genetics, 30(2), 1996, pp. 145-150
Citations number
34
Categorie Soggetti
Genetics & Heredity
Journal title
Current genetics → ACNP
ISSN journal
01728083
Volume
30
Issue
2
Year of publication
1996
Pages
145 - 150
Database
ISI
SICI code
0172-8083(1996)30:2<145:IAMAOT>2.0.ZU;2-#
Abstract
By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lact is we have isolated five nuclear mutants that were unable to grow on n on-fermentable carbon sources. The mutations were found to belong to t hree complementation groups. After functional complementation of the m utation in one of these mutants we have cloned the structural gene for cytochrome cl, named KlCYT1. This gene has been assigned to chromosom e VI and its nucleotide sequence exhibited 74.3% identity to the homol ogous gene of S. cerevisiae.