F. Kempken et W. Howad, MITOCHONDRIAL RNA EDITING IS SEQUENCE-SPECIFIC AND INDEPENDENT OF TRANSCRIPT ABUNDANCE IN SORGHUM-BICOLOR, Current genetics, 30(2), 1996, pp. 186-189
The DNA sequence which encodes the amino-terminal extension to the con
served core of the atp6-1 gene found in line IS1112C is absent from Tx
398. Sequences further upstream are present in Tx398, but at a differe
nt genomic location, The atp6-2 genes are present in similar copy numb
ers in IS1112C and A3Tx398, a near-isogenic line carrying the IS1112C
cytoplasm in a Tx398 background. However, transcript abundance of atp6
-2 in these lines is about ten-times higher than that of atp6-1. RNA e
diting of atp6-1 transcripts is identical to that of atp6-2 and theref
ore sequence specific. A single non-silent editing site in the unique
atp6-1 pre-piece sequence may indicate mitochondrial-guided RNA editin
g, While in Petunia the abundance and RNA editing of a transcript are
correlated, we show here that RNA editing is independent of transcript
abundance and is sequence specific in Sorghum.