SYNTHESIS AND RIBONUCLEASE-H RELATED BIOCHEMICAL-PROPERTIES OF ALPHA-ANOMERIC 2'-DEOXYPYRIMIDINE HOMOOLIGOMER AND ITS PHOSPHOROTHIOATE ANALOG

Authors
SHINOZUKA K YAMADA N NAKAMURA A OZAKI H SAWAI H
Citation
K. Shinozuka et al., SYNTHESIS AND RIBONUCLEASE-H RELATED BIOCHEMICAL-PROPERTIES OF ALPHA-ANOMERIC 2'-DEOXYPYRIMIDINE HOMOOLIGOMER AND ITS PHOSPHOROTHIOATE ANALOG, Bioorganic & medicinal chemistry letters, 6(15), 1996, pp. 1843-1848
Citations number
21
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
6
Issue
15
Year of publication
1996
Pages
1843 - 1848
Database
ISI
SICI code
0960-894X(1996)6:15<1843:SARRBO>2.0.ZU;2-2
Abstract
RNase H mediated degradation of RNA on the beta-DNA . RNA duplex was i nhibited by the presence of extra 2'-deoxypyrimidine homooligomer. The effect was dependent on the nature of the nucleobase, glycoside bond configuration, and the internucleotide linkage of the extra homooligom er used. Thus, alpha-anomeric 2'-deoxycytidylate phosphorothioate exhi bited the most potent inhibitory effect (> 40%) on RNase H. Copyright (C) 1996 Elsevier Science Ltd