ANEUPLOIDY DETECTION IN HUMAN SPERM NUCLEI USING PRINS TECHNIQUE

Rapid and specific identification of chromosomes can be attained in si tu using the PRimed IN Situ (PRINS) labelling technique. We have adapt ed this technique to mature human sperm in combination with a protocol for simultaneous decondensation and denaturation of sperm nuclei. Thi s strategy allowed us to obtain double labelling of human spermatozoa in a <2-hr reaction. In the present study, we report the estimates of disomy for chromosomes 3, 7, 10, 11, and 17 on 64,642 spermatozoa from 2 normal males. The incidences of disomy ranged from 0.28-0.34%. Ther e were no significant interindividual or interchromosomal differences in disomy rates. (C) 1996 Wiley-Liss, Inc.