T. Akema et al., ACUTE IMMOBILIZATION STRESS AND INTRAVENTRICULAR-INJECTION OF CRF SUPPRESS NALOXONE-INDUCED LH-RELEASE IN OVARIECTOMIZED ESTROGEN-PRIMED RATS, Journal of neuroendocrinology, 8(8), 1996, pp. 647-652
The present study was undertaken to evaluate the role and possible int
eraction of the endogenous opioid peptide (EOP) and corticotropin-rele
asing factor (CRF) in the acute stress-induced suppression of gonadotr
opin secretion in ovariectomized estrogen-primed rats. An intravenous
(i.v.) injection of naloxone (10 or 20 mg/kg), an EOP antagonist, sign
ificantly elevated serum luteinizing hormone (LH) levels within ID min
in non-stressed animals. The naloxone-induced LH release was complete
ly eliminated when tested 30 min after the onset of acute immobilizati
on, In a subsequent study, it was found that suppression of the naloxo
ne-induced LH release occurred as early as 5 min after the stress onse
t, and was still evident 60 min after the end of a 30-min period of im
mobilization. The effect of naloxone was restored 3 h after liberation
of the animal from the 30-min immobilization. An intraventricular (i.
c.v.) injection of CRF (1 or 5 mu g) also significantly suppressed, in
a dose-related manner, the effect of a subsequent i.v. injection of n
aloxone, However, an i.c.v. injection of alpha-helical CRF(9-41) (25 o
r 50 mu g), a CRF antagonist, prior to immobilization, could not inter
fere with the suppressive effect of stress on naloxone-induced LH rele
ase, These results suggest that both acute immobilization stress and C
RF can inhibit the LH secretory activity without mediation by EOP neur
ons, However, the stress-related suppression may involve non-CRF mecha
nism(s).